Effect of Staphylococcus sp. Bacteria Isolated from Salicornia Plant on Wheat Growth

Document Type : Complete scientific research article

Authors

1 ...

2 Soil Science and Engineering Dep., College of Agriculture & Natural Resources, University of Tehran,

Abstract

Background and objectives: Salinity is one of the most extensive processes of soil degradation, which limits the increase in the production of food products for more demand. Inoculation of plants with salt tolerant growth promoting bacteria with the ability to produce ACC-deaminase and indole acetic acid often decreases the negative effects of high salt concentration and improves plant growth parameters. Therefore, the aim of this study was to determine the effect of rhizosphere and endophytic salt tolerant bacteria on salinity isolated from rhizosphere and Salicornia roots on wheat growth parameters in different salinity concentrations.
Materials and methods: This experiment was conducted in greenhouse conditions in a completely randomized design with four levels of salinity: 0, 44, 77 and 110 mM NaCl and four levels of bacteria: no bacterial treatments (control) rhizosphere, endophytic and combined treatment (rhizosphere and endophytic) were designed and implemented in 3 replications. After harvest, the morphological and physiological characteristics of the wheat were measured. Data analysis was performed using SAS software.
Results: The results showed that plant yield was significantly affected by salinity. Increasing the concentration of NaCl from 0 to 44 mM did not have any effect on all growth parameters, and in some cases, it improved them. But with increasing from 44 to 77 and 110 mM, salinity had a negative effect on all growth parameters and had the highest negative effect in the concentration of 110 mM. . In the absence of salt stress, the presence of bacteria increased plant yield. In salt stress conditions, inoculation of bacterial isolates had a significant effect on plant growth and increased the dry weight of shoot (9.49-26.68%), root length (3.94 -27.47%), dry weight of Root (10-47.36%), dry weight of root ratio dry weight of shoot (19.41-20.69%) and reduction of leaf relative water content (1.2-11.71%), superoxide dismutase (7.63-15.8%) and Proline (12.5-33.33%) than control.
Conclusion: In general, inoculation of bacteria, in 7 of 13 growth parameters resulted in improvement. The reason for not having effect in the other 6 parameters can be attributed to the halotolerant bacterial and the relative resistance of the cultivar used by wheat. According to the results obtained in this study for use of growth promoting bacteria, salt tolerant plant isolated from Salicornia as a bio-fertilizer, to improve growth parameters, reduce the effects of salinity stress and increase the yield of wheat plant requires more studies (using high salinity levels and different wheat cultivars) is at the greenhouse level.

Keywords

References

1.A Kumar, D.S. 1998. Use of physiological indices as a screening technique for drought tolerance in oilseed Brassica species. Annals of Botany.81: 3. 413-420.
2.Ali, S., Charles, T.C., and Glick, B.R. 2014. Amelioration of high salinity stress damage by plant growth-promoting bacterial endophytes that contain ACC deaminase. Plant Physiology and Biochemistry. 80: 160-167.
3.Barra, P.J., Inostroza, N.G., Acuña, J.J., Mora, M.L., Crowley, D.E., and Jorquera, M.A. 2016. Formulation of bacterial consortia from avocado (Persea americana Mill.) and their effect on growth, biomass and superoxide dismutase activity of wheat seedlings under salt stress. Applied Soil Ecology 102(Supplement C): 80-91.
4.Bates, L.S., Waldren, R.P., and Teare, I. D. 1973. Rapid determination of free proline for water-stress studies. Plant and Soil. 39: 1. 205-207.
5.Beauchamp, C., and Fridovich, I. 1971. Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Analytical Biochemistry 44: 1. 276-287.
6.Bent, E., Tuzun, S., Chanway, C.P.,and Enebak, S. 2001. Alterations inplant growth and in root hormonelevels of lodgepole pines inoculatedwith rhizobacteria. Can. J. Microbiol.47: 1. 793-800.
7.Bharti, N., Yadav, D., Barnawal, D., Maji, D., and Kalra, A. 2013. Exiguobacterium oxidotolerans, a halotolerant plant growth promoting rhizobacteria, improves yield and content of secondary metabolites in Bacopa monnieri (L.) Pennell under primary and secondary salt stress. World J. Microbiol. Biotechnol. 29: 1. 379-387.
8.Chang, P., Gerhardt, K.E., Huang, X.D., Yu, X.M., Glick, B.R., Gerwing,P.D., and Greenberg, B.M. 2014. Plant Growth-Promoting Bacteria Facilitatethe Growth of Barley and Oats inSalt-Impacted Soil: Implications for Phytoremediation of Saline Soils. Inter. J. Phytoreme. 16: 11. 1133-1147.
9.Dodd, I.C., and Perez-Alfocea, F. 2012. Microbial amelioration of crop salinity stress. J. Exp. Bot. 63: 9. 3415-3428.
10.Donate-Correa, J., León-Barrios, M., and Pérez-Galdona, R. 2005. Screening for plant growth-promoting rhizobacteria in Chamaecytisus proliferus (tagasaste), a forage tree-shrub legume endemic to the Canary Islands. Plant and Soil.
266: 1. 261-272.
11.Etesami, H. 2018. Can interaction between silicon and plant growth promoting rhizobacteria benefit in alleviating abiotic and biotic stresses in crop plants? Agriculture, Ecosystems and Environment. 253: 98-112.
12.Etesami, H., and Beattie, G.A.2017. Plant-Microbe Interactions in Adaptation of Agricultural Crops to Abiotic Stress Conditions. In Probiotics and Plant Health, 163-200 (Eds V. Kumar, M. Kumar, S. Sharma and R. Prasad). Singapore: Springer Singapore. Pp: 163-200.
13.FAO. 2016. Food and Agriculture: Key to Achieving the 2030 Agenda for Sustainable Development. FAO: 32.
14.Glick, B.R. 2014. Bacteria with ACC deaminase can promote plant growth and help to feed the world. Microbiological Research. 169: 1. 30-39.
15.Godfray, H.C.J., Beddington, J.R., Crute, I.R., Haddad, L., Lawrence, D., Muir, J.F., Pretty, J., Robinson, S., Thomas, S.M., and Toulmin, C. 2010. Food Security: The Challenge of Feeding 9 Billion People. Science 327(5967): 812.
16.Gontia, I.K., Kumari Schmid, M., Hartmann, A., and Jha, B. 2011. Brachybacterium saurashtrense sp. nov., a halotolerant root-associated bacterium with plant growth-promoting potential. Inter. J. System. Evol. Microbiol. 61: 12. 2799-2804.
17.Goswami, D., Dhandhukia, P., Patel, P., and Thakker, J.N. 2014. Screening of PGPR from saline desert of Kutch: growth promotion in Arachis hypogea by Bacillus licheniformis A2. Microbiol Research. 169: 1. 66-75.
18.Hasanuzzaman, M., Nahar, K., and Fujita, M. 2013. Plant response to salt stress and role of exogenous protectants to mitigate salt-induced damages. In ecophysiology and responses of plants under salt stress, 25-87 (Eds P. Ahmad, M. M. Azooz and M. N. V. Prasad). New York, NY: Springer New York.Pp: 25-87.
19.Hayat, R., Ali, S., Amara, U., Khalid, R., and Ahmed, I. 2010. Soil beneficial bacteria and their role in plant growth promotion: A review. Annals of Microbiology, 60: 4. 579-598.
20.Houba, V.J.G. 1988. Soil and plant analysis: a series of syllabi. Wageningen: Wageningen Agricultural University, Department of Soil Science and Plant Nutrition.
21.Jha, B., Gontia, I., and Hartmann, A. 2012. The roots of the halophyte Salicornia brachiata are a source of new halotolerant diazotrophic bacteria with plant growth-promoting potential. Plant and Soil. 356: 1. 265-277.
22.Joshi, R., Mangu, V.R., Bedre, R., Sanchez, L., Pilcher, W., Zandkarimi, H. and Baisakh, N. 2015.Salt adaptation mechanisms of halophytes: Improvement of salt tolerance in crop plants. In elucidation of abiotic stress signaling in plants: Functional Genomics Perspectives, 2: 243-279.
23.Kavi Kishor, P.B., Sangam, S., Amrutha, R.N., Sri Laxmi, P., Naidu, K.R., Rao, K.R.S.S., Rao, S., Reddy, K.J., Theriappan, P., and Sreenivasulu, N. 2005. Regulation of proline biosynthesis, degradation, uptake and transport in higher plants: Its implications in plant growth andabiotic stress tolerance. Current Science. 88: 3. 424-438.
24.Larsen, H. 1986. Halophilic and halotolerant microorganisms-an overview and historical perspective. FEMS Microbiology Letters. 39: 1-2. 3-7.
25.Mapelli, F., Marasco, R., Rolli, E., Barbato, M., Cherif, H., Guesmi, A., Ouzari, I., Daffonchio, D., and Borin, S. 2013. Potential for plant growth promotion of rhizobacteria associated with Salicornia growing in Tunisian hypersaline soils. BioMed Research International. 2013: 13.
26.Mayak, S., Tirosh, T., and Glick, B.R. 2004. Plant growth-promoting bacteria that confer resistance to water stress in tomatoes and peppers. Plant Science 166: 1. 525-530.
27.Munns, R., and Tester, M. 2008. Mechanisms of salinity tolerance. Annual Review of Plant Biology.
59: 1. 651-681.
28.Penrose, D.M., and Glick, B.R. 2003. Methods for isolating and characterizing ACC deaminase-containing plant growth-promoting rhizobacteria. Physiologia Plantarum. 118: 1. 10-15.
29.Rojas-Tapias, D., Moreno-Galván, A., Pardo-Díaz, S., Obando, M., Rivera, D., and Bonilla, R. 2012. Effect of inoculation with plant growth-promoting bacteria (PGPB) on amelioration of saline stress in maize (Zea mays). Applied Soil Ecology. 61: 264-272.
30.Rozema, J., and Schat, H. 2013.Salt tolerance of halophytes, research questions reviewed in the perspective of saline agriculture. Environmental and Experimental Botany 92(Supplement C): 83-95.
31.Rueda-Puente, E.O.C.C., Thelma Díaz de León-Álvarez, J.L., Preciado-Rangel, P., and Almaguer-Vargas, G. 2010. Bacterial community of rhizosphere associated to the annual halophyte Salicornia bigelovii (Torr.). Terra Latinoamericana. 28: 4. 345-353.
32.Sandhya, V., Ali, S.Z., Grover, M., Reddy, G., and Venkateswarlu, B. 2010. Effect of plant growth promoting Pseudomonas spp. on compatible solutes, antioxidant status and plant growth of maize under drought stress. Plant Growth Regulation. 62: 1. 21-30.
33.Sapre, S., Gontia-Mishra, I., and Tiwari, S. 2018. Klebsiella sp. confers enhanced tolerance to salinity and plant
growth promotion in oat seedlings (Avena sativa). Microbiological Research 206(Supplement C): 25-32.
34.Shetty, K.G., Hetrick, B.A.D., and Schwab, A.P. 1995. Effects of mycorrhizae and fertilizer amendments on zinc tolerance of plants. Environmental Pollution. 88: 3. 307-314.
35.Shukla, P.S., Agarwal, P.K., and Jha, B. 2012. Improved salinity tolerance of Arachis hypogaea (L.) by the interaction of halotolerant plant-growth-promoting rhizobacteria. J. Plant Growth Regul.31: 2. 195-206.
36.Singh, R.P., Jha, P., and Jha, P.N. 2015. The plant-growth-promoting bacterium Klebsiella sp. SBP-8 confers induced systemic tolerance in wheat (Triticum aestivum) under salt stress. J. Plant Physiol. 184(Supplement C): 57-67.
37.Singh, R.P., and Jha, P.N. 2017. The PGPR Stenotrophomonas maltophilia SBP-9 Augments resistance against biotic and abiotic stress in wheat plants. Frontiers in Microbiology. 8: 1945.
38.Sobhanian, H., Aghaei, K., and Komatsu, S. 2011. Changes in the plant proteome resulting from salt stress: Toward the creation of salt-tolerant crops? J. Proteom. 74: 8. 1323-1337.
39.Sowndhararajan, K., and Chul, S. 2012. In vitro antagonistic potential of Streptomyces sp. AM-S1 against plant and human pathogens.
40.Sperber, J.I. 1958. The incidence of apatite-solubilizing organisms in the rhizosphere and soil. Austr. J. Agric. Res. 9: 6. 778-781.
41.Szymanska, S., Borruso, L., Brusetti,L., Hulisz, P., Furtado, B., and Hrynkiewicz, K. 2018. Bacterial microbiome of root-associated endophytes of Salicornia europaea in correspondence to different levels of salinity. Environmental Science and Pollution Research. 25: 25. 25420-25431.
42.Szymańska, S., Płociniczak, T., Piotrowska-Seget, Z., and Hrynkiewicz, K. 2016. Endophytic and rhizosphere bacteria associated with the roots of the halophyte Salicornia europaea L. – community structure and metabolic potential. Microbiological Research. 192(Supplement C): 37-51.
43.Yuan, Z., Druzhinina, I.S., Labbe, J., Redman, R., Qin, Y., Rodriguez, R., Zhang, C., Tuskan, G.A., and Lin, F. 2016. Specialized microbiome of a halophyte and its role in helpingnon-host plants to withstand salinity. Sci Rep 6: 32467.
44.Zahir, Z.A., Ghani, U., Naveed, M., Nadeem, S.M., and Asghar, H.N.2009. Comparative effectiveness of Pseudomonas and Serratia sp. containing ACC-deaminase for improving growth and yield of wheat (Triticum aestivum L.) under salt-stressed conditions. Archives of Microbiology. 191: 5. 415-424.
Journal of Water and Soil Conservation
Volume 26, Issue 6
March and April 2020
Pages 179-196

Files

Share

How to cite

Statistics