Effect of water stress and humic acid foliar application on morpho-physiological characteristics of Satureja hortensis

Document Type : Complete scientific research article

Authors

1 Ph.D student of Crop Ecology, Lorestan University

2 Associated professor, Department of irrigation and soil physics, Soil and Water Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.

3 Ph.D student of soil science, Shahid Chamran University of Ahwaz

4 Assistant Professor, Department of Water Sciences and Engineering, Ahvaz Branch, Islamic Azad University, Ahvaz, Iran.

Abstract

Background and Objectives: Satureja hortensis plant is a medicinal, annual, Herbaceous belonging to the family Lamiaceae, which is used as a medicinal plant and spice, as well as fresh vegetable. Various plant species show a wide range of resistance to drought due to their physiological, morphological and biochemical adaptations. The use of various organic fertilizers, including humic acid, has been widely used to improve the quality and quantity of crops and gardens without harmful environmental effects. Hormonal effects and enhancement of the absorption of nutrients to enhance performance, especially under stress conditions, are the effects of humic acid is derived from humus and other natural sources and Growth stimulating bacteria. Considering the dry and semi-dry nature of a large area of Iran, it is necessary to save water; on the other hand, nutrition is important in the yield and quality of the effective materials of medicinal plants. Therefore, the purpose of this study was to investigate the effects of drought stress and humic acid on morphological and physiological characteristics of Satureja hortensis.
Materials and Methods: The experiment was a factorial based on a completely randomized design with four replications in greenhouse conditions in 2017. Four levels of irrigation water regulations 40, 60, 80 and 100% of field capacity and foliar application of humic acid in concentrations of 0, 150, 300 and 450 mg/L were applied. In the 6 to 8 leaves stage, Drought treatments were applied to the pots. Humic acid was applied in two stages in the desired concentrations. The first stage of spraying was when the plants were in the 4 to 6 leaves stage and the second stage was done two weeks after the first spraying. About 7 weeks after application of drought treatments, traits such as plant height, number of branches, number of leaves, leaf length, plant dry weight, proline, chlorophyll a, b and total, carotenoid and carbohydrates were measured.
Results: The results showed that the highest plant height, leaf number, number of branches and plant dry weight were obtained under irrigation conditions at 100% FC and spraying with 450 mg/l humic acid; While the highest leaf length, chlorophyll a, b and total, carotenoid and carbohydrate were obtained from irrigation treatment at 100% FC and 300 mg / l humic acid. Also, the highest proline content was obtained from irrigation treatments at 40% FC without humic acid application.
Conclusion: Satureja hortensis plant is susceptible to drought stress and with increasing stress and reaching 40% of FC, decrease in the amount of physiological and morphological traits of Satureja hortensis. Meanwhile humic acid had a positive and significant effect on these traits. Finally, by improving the absorption of nutrients and the development of Vegetative and reproductive parts, the physiological and morphological traits increased. The highest effect of humic acid treatment on drought stress of Satureja hortensis was in the treatment of 450 mg/l. The highest salinity production was obtained in irrigation treatment with 100% FC and 450 mg/l humic acid.

Keywords

References

1.Ahmad, Y.M., Shahlaby, E.A., and Shnan, N.T. 2011. The use of organic andinorganic cultures in improvingvegetative growth, yield characters andantioxidant activity of roselle plants(Hibiscus sabdariffa L.). Afr. J.Biotechnol. 10: 11. 1988-1996.
2.Aiken, G.R., McKnight, D.M., Wershaw,R.L., and Mac Carthy, P. 1985. Anintroduction to Humic Substances in Soil,Sediment, and Water. P 1-9, In: Humicsubstances in soil, sediment and water:
Geochemistry, isolation and characterization.(Eds. Aiken, G.R. et al.). WileyInterScience, New York.
3.Armand, N., Amiri, H., and Ismaili, A.2016. Interaction of methanol spray andwater-deficit stress on photosynthesis andbiochemical characteristics of Phaseolusvulgaris L. cv. Sadry. Photochem
Photobiol. 92: 1. 102-110.
4.Arnon, D.I. 1949. Copper enzymes inisolated chloroplasts. Polyphenoloxidasein Beta vulgaris. Plant Physiol. 24: 1-10.
5.Ayas, H., and Gulser, F. 2005. The effectof sulfur and humic acid on yieldcomponents and macronutrient contentsof spinach. J. Biol. Sci. 5: 6. 801-804.
6.Bates, S., Waldern, R.P., and Teare, E.D.1973 .Rapid determination of free prolinefor water stress studies. Plant and Soil.39: 205-207.
7.Delfin, S., Tognetti, R., Dsiderio, E., andAlvino, A. 2005. Effect of foliarapplication of N and humic acids ongrowth and yield of durum wheat. AgronSustain Dev. 25: 183-191.
8.Emarat Pardaz, J., Hami, A., and Gohari,Gh. 2016. Evaluation of GrowthCharacteristics and Function of SaturejaHortensis L. Essential Oil under Salinityand Zinc Foliar Application. J. Agric.
Know. Sust. Prod. 26: 3. 141-131.(In Persian)
9.Erkossa, T., Stahr, K., and Tabor, G.2002. Integration of organic andinorganic fertilizers: effect on vegetableproductivity. EIAR. 82: 247-256.
10.Eskandari, M. 2013. Growth parametersand changes in the essential oil contentof Satureja bachtiarica Bunge under theinfluence of 28-hobrosrinolide anddrought stress. Research on medicinalplants and aromatic herbs of Iran.29: 1. 186-176. (In Persian)
11.Esmailpour, B. Jalilvand, P., and Hadian,J. 2013. Effect of Drought Stressand Mycorrhizal Fungus on SomeMorphophysiological Traits andPerformance Satureja (Satureja HortensisL.). J. Agric. Ecol. 5: 2. 177-169.(In Persian)
12.Hu, Y., and Schmidhalter, U. 2005.Drought and salinity: A comparison oftheir effects on mineral nutrition ofplants. J. Plant Nutr. 168: 541-549.
13.Jacob, H., and Clark, G. 2002. Methodsof Soil Analysis. Part IV PhysicalMethod. Soil Science Inc. Madison,Wisconsin, USA. 1692p.
14.Jing-Min, Z., Shang-Jun, X., Mao-Peng,S., Bingyao, M., Xiu-Mei, C., andChunsheng, L. 2010. Effect of HumicAcid on Poplar Physiology andBiochemistry Properties and Growthunder Different Water Level. J. SoilWater Cons. 6: 1-42.
15.Keles, V., and Oncel, I. 2004. Growth andsolute composition on two wheat speciesexperiencing. CSSA. 40: 470-475.
16.Khalid, K.A. 2006. Influence of waterstress on growth, essential oil andchemical composition of herbs (Ocimumsp.). Int. Agrophysics. 20: 4. 289-296.
17.Liu C., Cooper, R.J., and Bowman, D.C.1998. Humic acid application affectsphotosynthesis, root development andnutrient content of creeping bentgrass.Horticulture Science. 33: 6. 1023-1025.
18.Mokhtari, A., and Baradaran, R. 2010.Effect of Drought Stress on SomeGrowth Indices of Satureja Hortensis,Regional Conference-E Ecophysiologyof Crops. Shushtar. Pp: 1-3. (In Persian)
19.Nouri, M., Asadi, P., DehghanRahimabadi, A., and Golchin, A. 2014.The Effect of Drought Stress andNitrogen Fertilizer Levels on Growthand Essential Oil of Savory (Saturejahortensis L.). Bull. Env. Pharmacol.Life Sci. 3: 10. 71-77.
20.Peleg, Z., Apse, M.P., and Blumwald, E.2011. Engineering Salinity and WaterStress Tolerance in Crop Plants: GettingCloser to the Field. Advances inBotanical Research. 57: 405-443.
21.Sabouri, F., Siroosmehr, A.R., andGorgini Shabankareh, H. 2017. Effect ofirrigation regimes and humic acidsolution on some morphological andphysiological characteristics of Saturejahortensis. Iran. J. Plant Biol. 9: 34. 13-24.(In Persian)
22.Sanjari, M., Siroosmehr, A.R., andFakheri, B.A. 2015. Effect of DroughtStress and Humic Acid on SomePhysiological Properties of Sour Tea. J.Agric. 17: 2. 403-414. (In Persian)
23.Sanjari, M., Siroosmehr, A.R., andFakheri, B.A. 2016. Effect of DroughtStress and Humic Acid on
Morphological Traits, Performance andAnthocyanin Levels of Sour Tea(Hibiscus sabdariffa L). Agric. Ecol. J.8: 3. 346-358. (In Persian)
24.Sudeyazadeh, H., Shamsai, M.,Tajamolian, M., MirmohammadiMeybodi, M., and Hakimzadeh, M.
2016. Effect of drought stress on somemorphological and physiological traitsof Satureja hortensis. J. Proc. PlantFunc. 5: 15. 1-12. (In Persian)
25.Thomas, M.T., and Gausling, T.2000. Morphological and physiologicalresponses of oak (Quercus petraea andQ. robur) to moderat drought. Ann. For.Sci. 57: 325-333.
26.Turkmen, O., Demir, S., Sensoy, S., andDursun, A. 2005. Effect of arbuscularmycorrhizal fungus and humic acid onthe seedling development and nutrientcontent of pepper grown under saline soilconditions. J. Bio. Sci. 5: 5. 568-574.
Journal of Water and Soil Conservation
Volume 26, Issue 1
May and June 2019
Pages 219-232

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